At MicroBios, we are committed to providing our customers with the industry’s best laboratory and scientific consulting services. We pride ourselves on the quality of our support and care, and on the success of our customers using our services.
MicroBios can provide individuals, local/national companies as well as government agencies with a broad range of laboratory services. Our biotechnological services can help you protect your intellectual property and assist you in the development of new technologies. Please contact MicroBios to inquire about any of the following services:
- Cell Culture Testing and Handling
- DNA Cloning
- Protein Engineering
- Heterologous Protein Production
- Protein Purification
- Protein Characterization
- Fermentation Process Optimization and/or Design
- Specialized Media Development and Optimization
- Recombinant and Non-recombinant Strain Improvement
Cell Culture Testing and Handling
MicroBios offers a wide range of prokaryotic and eukaryotic cell culture testing and handling services. These services meet the needs of researchers who are looking for results without the difficulties of internal handling. Our scientists specialize in designing custom studies, but will also follow a protocol prescribed by our customers. The experts at MicroBios have years of experience working with in vivo systems and would be glad to help you further your research goals.
The list below shows a selection of cell culture testing and handling services we provide. If you are interested in any of these services, contact us by e-mail to send us your requirements. Our staff will gladly evaluate them and provide you with an estimate as soon as possible.
Standard Cell Culture Services
- Gram staining and enumeration of culturable bacteria
- 16S rRNA gene sequencing / ribotyping
- Fatty acid profiling
- Microscopy characterization (brightfield, phase-contrast, confocal laser, SEM, TEM)
- Physiological characterization
- Antibiotic susceptibility testing
- American Type Culture Collection (ATCC) strain submission
- Strain procurement
- Storage of frozen stocks of microorganisms (short-term, long-term at -80°C)
- Toxicology and immunological studies
If your needs are not included in our standard testing services, contact us by e-mail to send us your needs. Our staff will evaluate all inquiries and promptly provide an estimate.
DNA Cloning
Our DNA cloning services provide the means to turn DNA sequences containing open reading frames (ORFs) into expression-ready clones. Open reading frames from full-length genes are amplified and cloned into an appropriate expression vector.
Protein Engineering
Working closely with academic partners (including Cornell University in Ithaca, NY), MicroBios has developed and continuously enhances a host of cutting edge proprietary biomolecular engineering techniques. The aim of Protein Engineering is to modulate and optimize specific protein properties.
Examples of modulated protein properties include:
- Function
- Solubility
- Resistance to Proteolytic Degradation
- Ability to Crystallize
- Transport
- Bioavailability
- Targeting
- Therapeutic Potential
Heterologous Protein Production
MicroBios’s Protein Production services include the following:
- Expression (prokaryotic and eukaryotic)
- Protein purification
- Quality assurance
Our experts will assist you in selecting the best possible expression system to meet your specific needs. Wherever applicable, MicroBios operates on a “fee-for-success” basis for maximum customer peace of mind.
Protein Purification
Our experience with methods of denaturation/refolding and reconstituting proteins in their active state means that we can assist you at each stage of the the purification process. We use a variety of separation methods to ensure the highest purity of your target protein, in whatever amount you require. Upon project completion, our specialists assess the final product according to your specifications (see Protein Characterization below).
Protein Characterization
Customers may submit protein samples to MicroBios for an assessment that includes any or all of the following:
- Gel Electrophoresis (SDS-PAGE, Native, IEF, 2-D) and Coomassie blue or silver staining
- Protein concentration determination
- Western immunoblotting
- Absorption spectrum measurement
- N-terminal sequencing
- Mass spectrometry
- Solution NMR spectroscopy
- Enzyme activity assay
Fermentation Process Optimization and/or Design (Up to 10-Liter Working Volume)
MicroBios uses factorial design in performing fermentation development at the 10-liter scale and in computer-controlled bioreactors. Careful statistical design is used in every process development project to achieve a rational and efficient approach. Whenever possible, defined, animal component-free growth media are employed.
Specialized Media Development and Optimization
MicroBios scientists can develop media formulations specifically suited to your working cell lines and expression systems. We can also formulate nutrient supplements to your specifications for customized bioreactor feed strategies.
